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Analysis Basics

  • RF-20A Fluorescence Detector Basics and Applications
  • A fluorescence detector is one of the typical detectors in an HPLC analysis. Its operational principle and characteristics enable a highly sensitive and selective analysis compared to a UV absorbance detector which is the first choice of detector in HPLC. Although the number of compounds that can be fluorescently detected is limited, a derivatization makes the technique applicable to a wide range of analyses. This leads to a highly sensitive quantitative analysis in many fields such as pharmaceutical, food, and environmental markets. In this report, the principle of the fluorescence detector and its advantages are described. Additionally, example analyses of Shimadzu fluorescence detector RF-20AXS will be introduced to showcase its unique temperature control function (e.g., cooling).

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