In fractionation purification using SFC, the target compounds are recovered in high concentrations in a concentrated organic solvent, which saves time not only during analysis, but also during post-processing after preparative tasks are complete. The Nexera UC Prep maximizes its output with its high recovery rates and ability to carry out continuous preparative work that further shortens the user’s waiting time while maximizing the rate of fractionation output.
High recovery rates
In preparative SFC, one factor that results in lower recovery rates is increased dispersion of the eluant when the CO2 returns suddenly from a supercritical to a gaseous state. The Nexera UC Prep’s patented gas-liquid separator, the LotusStream separator, successfully reduces sample dispersion and carryover, while also achieving high recovery rates. These high recovery rates can be obtained regardless of flow rate or modifier concentration, even for volatile compounds such as the fragrance linalool.
Comparison for 1% linalool
LotusStream separator (patented technology)
Decreases flow speeds without increasing the tubing diameter by splitting flow through multiple flow channels. The CO2 is discharged externally while the liquid travels along the column and drips directly below without dispersing the eluate.
Prep Solution enables a seamless preparative workflow
The dedicated software, Prep Solutions, ensures that it is simple to scale up from an analytical to a preparative workflow, and makes it easy to configure parameter settings. It greatly increases the efficiency of preparative purification workflows.
Easy to understand even for first-time users
The parameter settings in Prep Solutions have been kept as simple and intuitive as possible, so that all users can operate the system without confusion. This also avoids the risk of wasting samples through human error.
A single analysis prior to fractionation confirms the peak elution positions. Analysis can be started by simply inputting basic parameters in the three onscreen tabs.
The chromatogram obtained from the single analysis can be displayed in the simulation window, so that fraction ranges can be selected by eye using the mouse. These settings can be applied to methods automatically.
Samples are fractionated based on the user-selected parameters. The fraction range is displayed on the chromatogram, which can be checked in real time.
Parameter settings for fractionation and injection can be adjusted during stacked injection (“on-the-fly” function).
Compact, benchtop design
This space-saving benchtop model includes a carbon dioxide pump that does not require an external chiller (cooling system for the heat source). In addition, one unit can handle multiple flow volumes, lowering installation costs.
Benchtop system that can be installed anywhere
Usually a chiller is required to cool the solvent delivery pump when pumping CO2 at high flow rates. However, the Nexera UC Prep features a compressor-type cooling unit, reducing the size of the system and allowing it to be installed anywhere. Its footprint is equivalent to an analysis-scale SFC system.
Choose the optimal configuration for your application
Stacked Fraction System: supports injection volumes up to 20 mL for high flow rates
This system is optimized for high preparative flowrates that involve repeatedly injecting compounds which may contain several components. It can be optimized not only for chiral compounds but also achiral compounds. The FRS-40SF unit includes both injector and fraction collector functionality, so that the same unit can be used for repeated sample injections and gram-level preparative work. It supports injection volumes up to 20 mL* and ten recovery columns. Compatible with flow rates from 10 to 150 mL/min and 10 to 30 mm I.D. columns. *optional
Multi-Fraction System: for multiple peak fractionation of impurities and natural substances
This system is suitable for preparative tasks involving samples with many peaks detected, such as impurities in pharmaceuticals. Volumes of up to 2 mL* can be injected using an autosampler that holds up to 162 samples (using 1.5 mL vials). Three rack types can be selected for the FRC-40 fraction collector, which can recover up to 540 fractions (using 10 mL vials). With support for flow rates from 10 to 150 mL/min, columns with an internal diameter from 10 to 30 mm can be used. *optional
Analytical Fraction System: analysis & low flow rate compatibility in one system
This system is intended for analysis-scale flowrates for applications that only require fraction volumes up to a few mg (used to check synthesis for example). By connecting an FRC-40 SF fraction collector to the Nexera UC system, the system can seamlessly perform everything from analysis to preparative work. With a flow rate range of up to 5 mL/min, analysis-scale columns can be used.
Stacked Fraction System
Analytical Fraction System
Flow rate range
Length：up to 250mm
Length：up to 250mm
Length：up to 250mm
No. of samples processed: 1
Max. injection volume: 2 mL
（optional: 20 mL）
No. of samples processed:
252 (with 1 mL sample vial plate)
162 (1.5 mL sample vial plate)
84 (4 mL sample vial plate)
36 (10 mL sample vial plate)
72 (microtube plate)
288 (96-well microplate)
1,152 (384-well microplate)
Max. injection volume: 500 μL