Biotage Apps



Microwave Assisted Organic and Peptide Synthesis of a Non-natural Arginine Residue And Incorporation Into a Cyclic Peptide Mupain-1 Analogue

​The cyclic peptide mupain-1 (CPAYSRYLDC) is an inhibitor of the cancer-related urokinase-type plasminogen activator. Here we show the synthesis of a non-natural arginine building block precursor using MAOS.

Microwave Assisted Synthesis of Peptide Nucleic Acid (PNA) Oligomers Using Biotage® Initiator+ Alstra™

PNA oligomers have interesting properties such as their increased thermal and chemical stability and resistance to enzymatic degradation which drives their use in various molecular biology, molecular diagnostic, microarray, biosensor and antisense applications. In this study we synthesize two PNA oligomers on a small scale using the Biotage® Initiator+ Alstra™ microwave peptide synthesizer.


Suzuki Coupling on Solid-Phase using Microwave Synthesis

Solid-Phase Suzuki couplings using microwave irradiation have been performed. The reaction times were considerably shorter than those required using conventional heating conditions.


Flash Chromatography

Cannabis extract purification using orthogonal flash column chromatography

​For some cannabis-based product developers reversed-phase chromatography has become the analytical tool of choice for determining the extract content profile as well as for purification of specific cannabinoid compounds. However, the extracts often contain many other compounds which reduce load capacity and purity of the product(s) of interest and then require even more extensive clean-up..

Environmental Sample Clean-up on Isolera™ by Size Exclusion Chromatography

This application note outlines how to use the Isolera system to perform a size-exclusion separation as part of a sample clean up method defined by the EPA, a key sample preparation step in environmental laboratories.

Green Solvents: Substitution for DCM-MeOH

This application note discusses ways to reduce the use of chlorinated solvents by using alternative solvent systems for flash column purification.


Extrahera Sample Prep Automation

Comparison of Biotage Extrahera vs Manual Sample Processing Using Vacuum Manifold: Extraction of Beta Blockers from Plasma Using ISPLUTE

This product sheet compares automated sample preparation using the Biotage® Extrahera™ to an equivalent manual method utilizing a vacuum manifold. A selection of beta blocker drugs were extracted from pooled stripped plasma using a supported liquid extraction procedure.

Ultra-Sensitive Method for the Determination of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 in Serum Using Supported Liquid Extraction Prior to LC-MS/MS

This application note describes a protocol for the extraction of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 metabolites from serum using supported liquid extraction prior to LC-MS/MS detection. A calibration range between 5 and 500 pg/mL is demonstrated using a starting volume of 0.25 mL of serum. Sensitivity is maximized through the use of a simple PTAD derivatization and formation of a methylamine complex. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.

Extraction of Methylmalonic Acid from Serum Using ISOLUTE® PPT+ Protein Precipitation Plates Prior to LC-MS/MS Analysis

This application note describes a simple ISOLUTE® PPT+ protocol for the extraction of methylmalonic acid (MMA) from serum, demonstrating high, reproducible analyte recoveries with low protein content in the extracts. ISOLUTE PPT+ Protein Precipitation plates offer a fast and extremely simple sample preparation approach. Serum proteins are efficiently removed from the sample using an in-plate, ‘solvent first’, protein crash and filter procedure, which is easily automated using the Biotage Extrahera system. Details of the automated procedure and data comparing manual and automated method performance are included.

For Research Use Only. Not for use in diagnostic procedures.

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